90 Negative Correlation in Oenothera Hybrids 



(No. 1231), divisible into a spotted and an unspotted group, in the ratio 

 103 : 94. No nanella-plsints were seen, and all were similar in morpho- 

 logical features to 0. rubrinervis, as shown in the second row of rosettes 

 from the top of Plate V, though in the 10-weeks-old rosettes about 

 two-thirds of the unspotted group had somewhat broader, darker green 

 leaves than the remaining one-third. Later, however, this distinction 

 could no longer be seen ; the broader- and narrower-leafed groups were 

 kept separate throughout their development, but no distinction was 

 visible between them in the mature plants. With the exception of 

 three plants which were slightly divergent in characters of foliage and 

 branching, all were of the same vegetative form, and indistinguishable 

 in this respect from the (a) group of selfed 0. rubricalyx. In pigmenta- 

 tion, however, a striking situation was presented. The unspotted 

 rosettes developed into plants of the same type as the corresponding 

 group in the selfed 0. rubricalyx family, having greenish stems and 

 brilliantly pigmented buds of the rubricalyx-type. The spotted rosettes, 

 on the other hand, produced brilliantly red-pigmented stems, with buds 

 of the Lamarckiana-type of pigmentation, the cones being merely pink 

 in longitudinal bands of greater or lesser width, and the hypanthia 

 green. No plant of the latter group had buds as strongly pigmented 

 as in 0. rubrinervis, the male parent, and the minus variations ranged 

 to cones nearly, though not quite, completely free from anthocyan. 



Oenothera rubrinervis x rubricalyx F^ 

 Pedigree Nos. 1123(8) x 11410(1) = 1233. 



Seeds from this cross produced 152 plants which were likewise 

 clearly separable into a spotted and an unspotted group, but with no 

 other apparent distinction. Two-months-old rosettes are shown in the 

 second row from the bottom of Plate V. The two groups consisted of 

 62 spotted and 89 unspotted rosettes, 1 having died unclassified. Plants 

 of both groups had red on the under side of petioles and leaf-blades, 

 especially when the latter were going into decline. Several plants 

 which had one or two obscure red spots or fine red specks, but proved 

 on their subsequent development to belong to the unspotted group, are 

 included with that group in the ratio given above. One plant which 

 was marked on May 2 as spotted, was found two weeks later without 

 spots, and was transferred to the unspotted group, where its identity 

 was lost. Among the adult plants one individual from the unspotted 



