94 Negative Correlation in Oenothera Hybrids 



stage. Two had heavier buds than the rest, nearly like those of pure 

 rubrinervis in size and form, but each of these had greenish stems and 

 rubricalyx-colored buds ; they are included among the number referred 

 to group (c) below. Aside from these two plants and several specimens 

 which failed to bloom, the 78 plants which had been set into the field 

 were referable to three phenotypes, indistinguishable from those pre- 

 sented by the reciprocal family. The several types of pigmentation 

 were distributed in the following proportions : 



(a) 7 [9] 1 with dull, dark-red stems and buds devoid of anthocyan. 



(6) 17 [22] with brilliant red stems, pink cones and green hypanthia. 



(c) 51 [82] with greenish stems and intensely red hypanthia and 

 cones. 



The relation of these groups to the rosette-characters of the same 

 family are the same as in the reciprocal family ; both (a) and (6) were 

 completely included among the spotted rosettes, while (c) included all 

 the unspotted rosettes and 26 (i.e. essentially 50 per cent) of the 

 spotted ones. 



Similar phenomena in other Oenothera-crosses. 



The negative correlation between the redness of the stems and that 

 of the buds, which has been so strikingly manifested in these rubricalyx- 

 crosses, is by no means limited to the combinations of 0. rubricalyx. In 

 an extensive series of crosses between 0. Lamarckiana and several 

 biotypes of 0. cruciata, glimpses of the same inverse relation in the 

 distribution of the red pigmentation, have been frequently seen. The 

 results of the latter crosses will be published in detail in another place, 

 and only several examples of many that might be presented will be 

 introduced here to illustrate the criss-cross distribution of the red 

 pigment on the stems and buds. The forms of 0. cruciata have green 

 buds and usually more or less strongly reddened stems. 0. Lamarckiana 

 has pink cones and green hypanthia associated with only moderate 

 reddening of the stems, the stems having a degree of red-pigmentation 

 similar to that of the above-described " greenish "-stemmed plants 

 among the rubricalyx-hy'brids. A cross between a certain elementary 

 form of 0. cruciata $ and 0. Lamarckiana produced in the F l 

 (Fed. No. 1140) four types characterized as follows with respect to 

 pigmentation : 



(a) 2 [2] 1 with red stems and buds entirely green. 

 1 See footnote on p. 92. 



