320 FRAGMENTS OF SCIENCE. 



boiling for five minutes, or even less, would be sure to 

 destroy them all; in other localities the diffused germs 

 are so obstinate, that many hours' boiling would be 

 requisite to deprive them of their power of germina- 

 tion. The absence or presence of a truss of desiccated 

 hay would produce differences as great as those here 

 described. The greatest endurance that I have ever 

 observed and I believe it is the greatest on record 

 was a case of survival after eight hours' boiling. 



As regards their power of resisting heat, the in- 

 fusorial germs of our atmosphere might be classified 

 under the following and intermediate heads: Killed in 

 five minutes; not killed in five minutes but killed in 

 fifteen; not killed in fifteen minutes but killed in 

 thirty; not killed in thirty minutes but killed in an 

 hour; not killed in an hour but killed in two hours; 

 not killed in two but killed in three hours; not killed 

 in three but killed in four hours. I have had several 

 cases of survival after four and five hours' boiling, some 

 survival after six, and one after eight hours' boiling. 

 Thus far has experiment actually reached; but there is 

 no valid warrant for fixing upon even eight hours as 

 the extreme limit of vital resistance. Probably more 

 extended researches (though mine have been very exten- 

 sive) would reveal germs more obstinate still. It is 

 also certain that we might begin earlier, and find germs 

 which are destroyed by a temperature far below that of 

 boiling water. In the presence of such facts, to speak 

 of a death-point of bacteria and their germs would be 

 unmeaning but of this more anon. 



' What present warrant,' it has been asked, ' is there 

 for supposing that a naked, or almost naked, speck of 

 protoplasm can withstand four, six, or eight hours' 

 boiling?' Regarding naked specks of protoplasm I 

 make no assertion. I know nothing about them, save 



