12th : The most advisable method for the sugar determina- 

 tion is the "Hot Water Digestion," and for the sake of uni- 

 formity and comparison of results from the different sections of 

 the United States, this method, and no other should be used in 

 the analytical work. The pulp should be as fine and uniform 

 as possible, and the use of boring machines is not recommended, 

 as too small a section is removed from each beet, and also be- 

 cause the sugar content of such a small section is not a fair 

 representative of the sugar content of the entire beet. 



13th : Several hundred beets produce a considerable quan- 

 tity of pulp. As it is impossible, for the average factory labora- 

 tory, to digest the entire amount, only a sample thereof will be 

 analyzed, and the selection of this sample is a feature of great 

 moment. The following method for securing this sample is 

 recommended : Divide the entire quantity in two parts 1 A and 

 B ; mix each thoroughly and then divide each again into Al, 

 A2 and Bl and B2. Then mix thoroughly Al with Bl and A2 

 with B2, then re-divide (Al and Bl) and (A2 and B2) and re- 

 peat this manipulation 10 to 15 times for about 15 minutes. 

 Finally about 5 to 7 samples are taken from this mixture and 

 the sugar in each one is determined. Since every seed variety 

 is from 3 to 5 times represented in a test field, from 15 to 30 

 individual determinations of sugar are thus obtained of each 

 by this method. 



14th : As errors in the reading of the polariscope are very 

 common, and occur even with the b'est chemists, the use of 4 

 to 5 samples is far superior to the use of one, though the latter 

 may represent 4 times the weight of any single one. The more 

 readings the smaller the margin of errors. 



In order to be able to form reliable conclusions of the re- 

 sults of these tests the greatest accuracy in connection with 

 every branch of the work in the field as well as in the laboratory 

 is of prime importance ; without it, more harm than good might 

 come from them. 



A supplementary edition treating the work in the labora- 

 tory in detail, and the method of calculating and determining 

 the results of the seed tests will be issued later in the season. 



Los Angeles, California, February, 1911. 



