588 APPENDIX 



INTRODUCTION 



General Histology of Body. Microscopic study of typical cells 

 and tissues. Desirable for students who have no previous histo 

 logical training. 



General Chemistry of Body. Inorganic Constituents. Water. 

 The percentage of water in flesh may be determined roughly by 

 placing a weighed piece of meat in a dessicator in a warm place and 

 weighing it at intervals until thoroughly dry. 



Inorganic Salts. A weighed piece of meat may be incinerated 

 under a hood and the ash weighed. The solubility of the ash in 

 water may be ascertained. Tests for chlorids with silver nitrate, 

 and for calcium with sodium oxalate solution may be applied. 



Organic Constituents. Carbohydrates. Dextrose is present i:i 

 honey and corn syrup. The test for its presence is by means cf 

 Fehling's Solution (see any laboratory manual of Organic Chem- 

 istry). The presence of a reddish precipitate in the test tube in 

 which mixed Fehling's Solution (alkaline copper tartrate) and 

 dextrose are combined and heated to boiling is the test. 



Glycogen is a constituent of oysters. Grind a raw oyster with 

 sand in a mortar. Extract with water. Add to a few drops of the 

 juice in a test tube a few drops of iodine solution (tincture may be 

 used). A mahogany-brown color is the test for glycogen. 



Lactose. If sweet milk is clotted with rennet and filtered the 

 whey contains milk sugar. Test with Fehling's solution as for 

 dextrose. 



Fats. Solubility of Fats. Shake a small piece of lard with so 

 ether in a test tube. Pour some of the solution on filter pa 

 and allow it to evaporate. If the ether dissolved the fat, a 

 grease spot will be left on the filter paper after the evaporatio 

 of the ether. 



Moisten a second filter paper with ether, allow it to evaporate. 

 Compare the two filter papers. The test may be repeated with 

 olive oil instead of lard. 



Test for Fats. Melt a small piece of lard in a test tube and to it 

 add a drop of dilute osmic acid. This acid turns fat black. 



Composition of Fats. Fats are compounds of fatty acids with 

 glycerin. To separate the constituents heat on a water bath a 

 small piece of lard with an alcoholic solution of caustic potash. 



" 



3. 



