THE SERUM TREATMENT OF GLANDERS. 6oT 



several birds which had received eight to twelve hypodermic or intra- 

 venous injections survived for some months. 



With serum thus obtained I treated during October and November, 

 1898, some guinea-pigs inoculated in the flank by scarification with 

 glanders virus taken from the horse. From twenty animals thus 

 inoculated on the 23rd September, 1898, I chose ten in which the local 

 lesions were well developed and practically of uniform intensity. All 

 showed ulcers with indurated bases, accompanied in most by inflam- 

 mation of the precrural lymphatic glands. Seven of these guinea-pigs 

 were injected every third or fourth day with thirty to eighty minims of 

 serum ; five were injected subcutaneously and two intra-peritoneally. In 

 four the injections were continued for five weeks (4th October to loth 

 November). In three the ulcer healed with fair rapidity, and in general 

 wasting appeared less marked than in the controls, but, like the latter, 

 the animals treated showed various secondary complications, such as 

 orchitis, abscess formation, and cutaneous ulcers ; and although three 

 months after inoculation one was still in fairly good condition and free 

 of apparent complications, it proved to be suffering from grave visceral 

 lesions. 



Two of the injected animals and a control died before the end of 

 the first month ; three injected animals and the two last controls 

 during the course of the second month ; and one of the two surviving 

 animals which had been treated on the seventy-fourth day. On 

 autopsy all these animals showed multiple visceral lesions, but 

 principally inflammation of lymphatic glands, granulations, and more 

 or less numerous nodules in the liver, spleen, or lungs. On post-mortem 

 examination the last of the animals, killed on the 27th December (after 

 ninety-five days), also showed lesions in the lymphatic glands, liver, 

 and spleen. 



I shortly transcribe two of the records concerning injected 

 animals : 



(i) Male guinea-pig, weighing thirteen ounces, inoculated on the 

 23rd September. On the 29th there was marked swelling of the scarified 

 region. On the 4th October a circular ulcer, the size of a threepenny 

 piece, had developed ; its base was cupped, greyish, dotted with red 

 points, and surrounded by an indurated zone ; the animal's weight 

 was twelve ounces and a half. 



On the 5th the first injection was made. On the loth the pre- 

 crural lymphatic glands were the size of a haricot bean, and a sar- 

 cocele had developed. The ulcer was stationary. On the i6th the 

 external lesions were in the same condition, but the animal was 

 already much wasted ; weight eleven ounces and three quarters. On 



