12 



The granule is either colourless or faintly yellow, 

 has a strong refractive power, and performs a lively 

 molecular movement wandering in the contents, and 

 generally found near one pole. Sometimes also 

 collapsed semi-lunar cells are found ; these, he says, 

 arc probably old varieties whose contents have been 

 evacuated. 



Although somewhat smaller than the yeast of beer, 

 Tokishige drew attention to the fact that it was a vege- 

 table organism resembling the yeast fungus, and 

 belonged to the order of saccharomyces. Fermi and 

 Aruch both disagree with him on this, and point out 

 that it does not ferment sugar. 



Staining 



This is said by almost every writer on the subject 

 to be difficult, and even by Nocard himself, who appears 

 to have been the first to stain the organism. He 

 recommends both the Gram-Nicolle and Gram-Weigert- 

 Kuhne methods, but since the disease was first recog- 

 nized in Ireland, during last October, I have to state 

 from information gained from an unpublished note 

 that Mettam was the first to show how easily really the 

 organism could be stained by either of these methods, 

 and I may here state that in doubtful cases staining is 

 invaluable for absolute accuracy and expedience in 

 diagnosing the presence or otherwise of the organism 

 in a specimen from a suspected v/ound or abscess. 



Nocard and Leclainche state that carbol-fuchsin 

 stains the organism after long contact, and they also 

 mention that Loeffler's methods for staining cilia may 

 also be resorted to. 



Recently the organism has been also stained by the 

 Claudius method, and for the convenience of my readers 



