1900] MICROSCOPICAL JOURNAL. 101 



antipodal cells in the embryo sac of grasses may best be 

 studied in grasses with large ovaries. They form either 

 before the fertilization of the egg or subsequeni to its 

 large cell complexes. For fixing, hot solutions of corro- 

 sive sublimate in alcohol and chromic acid were used 

 working up through the alcohol and into paraflBne. He 

 uses paraffine of two melting points 43° C. and a mixture 

 of IT C. and 54°C. The specimens are placed in the 43° C. 

 paraffine first and allowed to remain in it two days, then 

 transferred to the mixture of harder paraffine, and left in 

 this bath two days longer. The specimen should be kept 

 in paraffine at its melting point as near as possible. (Jour. 

 Appl. Mic. 3 : 718). 



Pores of Diatoms. —0. Muller who has investigated the 

 pores of Diatoms has made some rather interesting ob- 

 servations on some of the species with gelatinous pores: 

 in the genera Diatoma, Tabellaria, Grammatophora, Syne- 

 dra.Licmophora andFragilaria. In nearly all thesegenera 

 tha author was able to demonstrate the presence of pores 

 through which the mucilaginous plasms was issued. 



Onygena equina. — H. M. Ward has discussed a fun- 

 gus which occurs on organic substances like hairs and 

 hoofs. The ascospores when heated at 35° C. and over, 

 with digestive juices or when digestive juices are added to 

 the fluid, germinate. The optimum for germinations is 

 22°C. The chlamydospores also germinate in the presence 

 of digestive juices as well as without. (Pliilosophical 

 Trans. Roy. Soc. of London. Ser. B. 191:269-291. PI., 

 21-24). 



Inheritance of an Acquired Character in a Fungus. 

 Errera has found that in cultures of Aspergillus niger, the 

 conidia adapted themselves to concentration of a medium 

 and that this is transmitted to a second generation, that 

 is that the organisms were capable of growing more readi- 

 ly in the second generation than the first and these or- 



