1897J 



MICROSCOPICAL JOUIINAL. 



153 



bearing' bacillus which has not infrequently appeared in 

 media boiled for ten minutes on three consecutive days. 



STERILIZATION OF BOUILLON WITH ONE BOILING. 



7 c c 

 7 c. c 

 25 c. c 



7 c. c 

 7 c. c 

 7 c. c 

 7 c. c 



S 2 



30 mm 

 30 min 

 30 min 



30 min 

 30 min 

 30 min 

 30 min 



^°g 



Fermentation tubes withoa* 

 per cent, glucose. 



STERILIZATION OF AGAR WITH ONE BOILING. 



STERILIZATION OF TUBES OF AGAR CONTAINING A LAR- 

 GER gUANTITY FOR MAKING PLATE CULTURES. 



Dec. 29, 1896 

 Jan. 27. 1897 

 Feb. 5, 1897 

 Mar. 16, 1S97 

 Mar. 27, 1S97 



Apr. 6, 1897 



3 



26 

 15 

 35 

 43 



w -^ 



12 c. c 30 mm 



15 c. c 30 min 



15 c. c 40 min 



18 c c 30 min 



j8 c. c 30 min 



iS c c 30 rain 



7 

 6 

 7 

 7 





o Left at room temp, for lo days 



Spore bearing bacillus be- 

 longing to the B. subtilit 

 [group. 



