198 THE AMERICAN 3I0NTHLY [June 



iiirh, llu' loiif^-iludinal lines of Siiri7-ellai;etntna, and the secon- 

 dary striKture htliiiiia nervosa with the posta.tce stamp frac- 

 ture ; for a one-ei«j"hth inch or for hig-her powers up to the 

 owe-twelfth the dotted secondaries of Craspedodisciis elegans 

 in certain conditions. 



MICROSCOPICAL MANIPUL VTION. 



To Stain the Ringworm Fungus. — Adamson recom- 

 mends the following- method for permently staining" trich- 

 ophyton : — 1. Soak the hair in a 5 to 10 per cent solution of 

 caustic potash on a slide for ten to thirty minutes. 2 Wash 

 in 15 per cent alcohol in water. 3. Dry on slide, and in 

 the case of scales fix by passing through the flame. 4. 

 Stain fifteen to sixt}' minutes in aniline gentian violet made 

 in the usual wa\\ by adding- a few drops of saturated alco- 

 holic solution of g-entian violet to aniline water. 5. One to 

 five minutes in (Oram's iodine solution. 6. Decolorize in 

 aniline oil two to three hours or longer. 7. Remove super- 

 fluous aniline oil h\ blotting paper. Mount in Canada 

 balsam. — Phar. Jour. 



Frozen Sections.- Kthyl chloride might profitably be 

 employed in preparing- frozen sections for histologfical pur- 

 poses. The results thus far obtained have been exceed- 

 ing-ly satisfactory, and, while the method is somewhat 

 expensive, no accessory ap])aratus is required for the 

 microtome. 



Hamilton's method of pre])aring the tissues for freez- 

 ing- g^ives g-ood results. Another way of getting- the tissue 

 ready is that recently advised by J. Orth. One hundred 

 parts of Muller's fluid are mixed when wanted with ten 

 parts of formol. Small pieces of the tissue under examin- 

 ation are fixed and hardened in this solution in the incuba- 

 tor for three hours. At the K;\\y\ of tiiis time they are 

 removed and thoroughly washed, and alcohol is g-radually 

 added until tliev are ])laced in <»5 ])er cent alcohol. This 

 latter re-agfent must, ol course, be removed before the tis- 

 sue is frozen. If desired, after washing, the specimen 



