1897] MICROSCOPICAL JOUitiNAL. 221 



METII.liMOGLOBlX. 



Tlic t^peclrum of MetujL'moglobin is obtained by first 

 pI•t•l)al•in^• Oxy-IIaBmoglobiii cry.stfibs by treating- dog's 

 bb>o(l wiMi ether and .shaking it until it becomes ijiky, 

 then aUowiim- it to.stand in a cool place for an lionrorso, 

 at which time a iirm mass will be formed, due to the 

 crystals. Tlie motiicr liquid is separated from the crys- 

 tals by filtering througii muslin or linen, squeezing the 

 mass so as to obtain the crystals in as })ure a form as 

 possible. The crystals are dissolved in distilled water 

 and a dilute solution is examined with the spectroscope. 

 The two bantls oH Ox y-Hseniuglobin ap[)ear. A few drops 

 of potassium permanganate are added and the solution 

 gently warmed. If sufficient time has elapsed foi' the 

 oxidation of the ()\y HaMnoglobin, the two bands will 

 have disappeared and instead a single band in the red 

 near the line C between C and D. Nearly the entire spec- 

 trum is absoibed. Sometimes it is a little difficult to get 

 this band, but if the oxidation has taken place it will be 

 seen. In the experiment at hand I left the solution 

 until the next day, liefoi-e it would give the above 

 result. 



C.\HBOX-3I()XOXIDE HiEMOGLOBIX. 



If coal gas be jiassed through blood which has been 

 detibiiiiaUd, it will assume a (dierry-red color, the car- 

 bon uiMiioxide n[' the g;is liaviugdriveu off the oxygen of 

 ihi' ()w llaiiioglobiii and 1;:ken its place. TIk^ reducing 

 agenls h.ave no intluence upon tiiis new substance, it being 

 more stable than Oxy-lla'inoglobin. The two absorjttion 

 bands ai'e 'lenrei' to K than in the ( )xy-IIa'mogiobin 



sped rum. 



H.KMATIX. 



The re(l corpuscdes ar(^ composed of a [iroteid sti'onni 

 and a biownish pigment v;hirh is calbid ha'rnatin. The 

 iron is a ))art of the luematin. It can be obtained either 

 as the acid luematin or the alkaline hiematin. 



