1897] MICROSCOPICAL JOURNAL. 263 



14. Let alone and repudiate this un-American title F. R. 

 M. S. and make F. A. M. S. an equal or superior honor, 

 but let it be conferred only for philanthropic work done. 



15. Meet only at central points within easy access of 

 many members. 



16. To double the membership, halve the costof member- 

 ship. 



17. Treat the periodicals so fairly and liberally that they 

 will work for the society all the time. 



18. Banish narrowness, selfishness, cliques, cranks, 

 unworthy ambitions and decide to become a power throug-h 

 the actual benefit conferred on the public. 



19. For extremely technical papers which almost no one 

 can understand substitute larg-ely papers that educated 

 people can see some meaning- in. 



20. Show continually the usefulness and application of 

 the microscope to all branches of practical industry and 

 the advancement of human happiness. 



MICROSCOPICAL MANIPULATION. 



Staining the Tubercle Bacillus in Sections. — This can 

 easily be done by the methods recommended orig-inallv bv 

 Ehrlich and by Ziehl. Many slig-ht modifications in tech- 

 nical details have been introduced by a larg-e numbers of 

 workers, but the essential step by which the Bacillus tuber- 

 culosis can be differentiated from other bacilli consists in 

 the use of mineral acids, such as nitric or sulphuric acid. 

 When bacilli have been well stained with methvl-violet or 

 with fuchsin, it is found that certain dilutions of sulphuric 

 acid and nitric acid will rapidly remove the stain from all 

 known pathog-enic bacilli, with the exception of the bacilli 

 of tuberculosis and of leprosy, which are discolored very 

 much more slowly. The use of nitric acid is, however, 

 objectionable when one has to deal with delicate tissues, 

 and even sulphuric acid, diluted with six parts of water, 

 will cause a certain amount of distortion. For this reason 

 bacteriolog-ists have long- wished to find a method in which 

 the use of strong- acids was done away with. Dr. Borrel, 



