Mr. H. J. Carter on the Freshwater Infusoria of Bombay. 37 



place in Euglena*, and consider analogous to, if not the same 

 as, that described in the Characese and in Spirogyra {loc. cit.). 



It should be remembered that in obtaining the ovules of Eu- 

 glena viridis for development, they should not be forced out of 

 the organism, but swept off the sides of the vessel an hour or 

 two after the Eugleme, together with some of the water in which 

 they have been living, have been collected and set aside for set- 

 tlement. 



I have also met with another species of Amoeba undergoing 

 ovular development, viz. A. verrucosa, Ehr. (fig. 12), precisely 

 like tliat which I have already described ; the Amoeba perishing 

 as the ovules are developed, and ending in becoming a mere 

 ovisac (fig. 13). 



When first formed, the ovules, which are spherical, consist of 

 a hyaline capsule enclosing a sphere of glairy, refractive fluid, 

 like that of the ovules of Spvngilla and Euglena (fig. 13a); but 

 as they begin to develope, this glairy matter becomes trans- 

 formed into a granuliferous mucus which is spread over the 

 inner surface of the capsule (fig. 13 6); and finally the granules 

 present motion, whether of themselves or by aid of the mucus 

 in which they are imbedded, I am ignorant, for thus far only 

 have I seen the development; but I am inclined to think the 

 next stage consists in the whole ovule becoming polymorphic, 

 like the ovule of Spongilla. This Amoeba appears to nie — for I 

 have watched the development of a group for many months to- 

 gether — to he the adult of my A. quadrilineata , and therefore the 

 latter is not a new species. The formation and development of 

 the ovules took place in April, and the organism appears to 

 require at least nine mouths to come to maturity. 



At p. 236 I have stated that I had observed " Vorticellce de- 

 veloped singly from Acineta." This was from inference. I have 

 since been able to follow the gemmules thrown off from the 

 Acineta-fovm of Vorticellce through their subsequent develop- 

 ment, and in no case have seen them take on any other form 

 than that of Acineta. The young genunule at the moment of 

 its exit is, as Stein has stated, exactly like the bud on Vorticella, 

 but when pursued to its resting-place, I have always found it 

 end in becoming an Acineta ; so that this is not a true instance 

 of alternation of generation. Others, viz. Drs. Lachmannt and 

 CienkowskiJ, have arrived at tlie same facts; and these gentle- 

 men also doubt the transfornuitiou of Vorticella into Acineta. 

 Time w'ill prove whether they or Professor Stein are right in this 

 also ; in the meanwhile I incline to side with the latter. 



* Annals,, vol. xvii. pi. 9. figs. 11-11. f Idem, vol. xi.\. p. 2'Sb, 185/. 

 X Quart. Journal Microscop. Science. No. 18. p. 9'). 18.")7. 



