1895.] MICROSCOPICAL JOURNAL. 383 



and alcohol, section perpendicularly to surface. Stain when 

 free from gum, in picro-carmiue, mount in acid glycerine. 



Sutural Fibres. — To see these, prepare the cornea of a 

 plagiostomal fish, the skate for instance. 



Nerves. — Remove the cornea from a living frog, and 

 place it in the following liquids: Filtered fresh lemon 

 juice for five minutes; chloride of gold, forty minutes; 

 acetified water. After remaining in the last for from 

 twenty-four to forty-eight hours, the cornea has become 

 violet and the nerves are properly colored. Scrape off 

 the epithelium; examine in glycerine. 



Nerves of the Epitheliurn. — Impregnate several cornese 

 as described in the preceding paragraph. Mount one in 

 glycerine without scraping off" the epithelium. Put 

 another in strong alcohol for twenty-four hours; section 

 perpendicularly to the surface. If the hardening is not 

 sufficient to allow of sectioning, finish by gum and alco- 

 hol. The nuclei of the fixed cells appear only when the 

 cornea is dead. 



THE IRIS. 



Muscle. — To study the muscular fibres of the iris take 

 the eye of a white rabbit. Divide it into two segments, 

 anterior and posterior, separating them so that the ante- 

 rior shall contain the entire iris. Place this segment in 

 the i alcohol for 24 hours. Then carefully detach the 

 iris, brush both surfaces to remove the epithelium, stain 

 in picro-carmine, and mount in formic acid glycerine. 



Nerves. — Treat the iris of a white rabbit by lemon 

 juice and gold chloride, following the technique described 

 for the demonstration of the corneal nerves. 



The epithelium of the iris will be studied in the sec- 

 tions of the whole eye. 



CRYSTALINE LENS. 



To dissociate the fibres of the lens we will employ Max 

 Schultze's method. Place the organ in the following 



