18 THE AMERICAN MONTHLY [Jjimmry, 



Exp. 2 — «i. Veast in Pasteur's fluid was well ilcvelopetl, as 

 many as nine cells hein*j loumi in one colonv. (Fi*:;. 6.) 



Exp. 2 — fi. In Pasteur's fluid without sujjar, for the most part 

 unde\ elopetl, some with one or even two buds. (Fijj. J. i 



Exp. 2 — r. In water and sujjiar as man\ as four cells iu one 

 colony, though some were not huddetl at all. (Fij;. S.) 



Exp. 2 — </. In water, very tew were found to he budded at all. 

 thouj^h some hail a siujjle hud. (Pig- 9.) 



The third series of experiments was for the iMUjiose of deter- 

 mininjj the effect of lijjht and heat. All iu Pasteui- s fluid. 



Exp. 3 — n. In laboratory, 24 hours in light, about 18° C, the 

 yeast hail grown well, as high as six cells being found in one 

 colony. (Fig. 10.) 



Exp. 3 — 6. In Ladies' Hall. 24 hours in light, probably about 

 the same temperature, scarcely any difference was to be seen from 

 above, though none quite so highlv developed. 



Exp. .■5— c. In Ladies' Hall, same conditions as above, except 

 in the dark, was fully as well developed. 



Exp. 3 — (/. In oven 4 hours at 40° C, witliout light, tiic cells 

 had not budded so well ; some, however, having two buds. 



Exp. 3 — c. In light, at 0° C, very few cells were budded, and 

 those had onlv a single bud. 



The fourth series of experiments were with a view to establish- 

 ing the injuriousness of certain ingredients, as mercuric chloride, 

 and it was added in diflerent quantities. Pasteur's fluid was 

 used ; time, 22 hours ; temperature, 18° C. 



Exp. 4 — a. In Pasteur's fluid with no mercuric chloride, as 

 high as nine cells together. (Fig. 11.) 



Exp. 4--3. In Pasteur with i : lo.cxDO HgCL, a number of 

 cells had two buds, while most were undeveloped. (Fig. 12.) 



Exp. 4 — c. With I : 1000 of HgCl., , a small number of cells 

 had a single bud. (Fig. 13.) 



Exp. 4 — (/. With I : 100 of HgCl.j, scarcely a budded coll was 

 to be seen. (Fig. 14.) 



From these experiments we conclude that the method of repro- 

 duction is by a s\steni of budding; that light is not essential, and 

 that the most favorable temperature is considerably above o^ and 

 below 40° C ; also that certain chemical substances are favorable, 

 while others are deleterious to growth. 



Biological Laboratory, 



Hamline University, Dec. /8, iHgz. 



Arranged Butterfly Scales. — When we were in Paris, last 

 summer, Mr. George Cliflord, who is a microscopist and one of 

 the most genial Englishmen in that citv, presented us with a 

 slide representing a rooster, a hen, and five chickens, made by 

 arranging butterfly scales. The slide is the work of Mr. H. 

 Dalton. of Paiis, and has already occasioned much admiration in 

 Washington. — C W. S. 



