■M\ TlIK AMKUUAN MONTlll^V [Fol.nmry, 



cvcnluallv jjrow out in :i nuliatc maiiiuT tVoiu the sclcrotiiiin as 

 sIiMulor, cohirlcss, scptato liyplui; or s|)ort)j)lK)rt>s, each iiroiliiciiifj 

 at its apc\ a whorl ot" simple ov branched concatenate chains ot" 

 small, elliptical, olive spores that ajjiee in every particular with 

 the secotuiaiv spoies borne on the mvceliimi ot" the i^erniinatiii'jf 

 spores (Fig. 12). The spores prothiced by the sclerotia. when 

 placed on the leaves of tlie host-plant, produce the I [rlerospo- 

 riutn. 



Fijj. 6 represents two sporophores of the Hctcrosporium 

 al'ter remamin«; in water on a sliile for four days ; it will be ob- 

 served that two sleiuler filaments have developed, each bcarinoj a 

 fascicle of chan«je of spores similar to the secondary spores borne 

 on the tilament of j^erminatin*; spores. 



Finallv, if leaves infested with the Ilcleroaporiuni are ex- 

 nmineil in the autumn, blackish perithecia will in many instances 

 be toutul ; these bodies are subj^jlobose, slijrhtly attenuated up- 

 wards, furnished at the apex with a minute aperture, and when 

 mature have the inner surface covered with very short sporo- 

 phores, each bearin»j at its apex a minute body, reseml)lin<^ in 

 every respect the secondary spores of Ilctcrosporiiim spores. 

 These minute perithecia originate from mycelium closely re- 

 sembling that of Hctcrosporinni. but I have not seen any sug- 

 gestion of a resemblance to these bodies in any of my cultures, 

 neither have I succeeded in causing the spores of these structures 

 to germinate ; consequently their relationship or otherwise with 

 Hcternsporium is at present unknown ; it is certain, however, 

 that these bodies have no necessary control over the continued 

 tlevelopment of the Hcterosporium. — J. R. M. S. 



.Microscopical IVchniciue Applied to Histology. — I. 



i From the French of M. Rene Boneval.) 



[The following chapters are abstracts of M. Rene Honeval's 

 admirable ** A^ouveau Guide Pratique de Tcciinique A/icro- 

 scopiquc appliquce a V Ilistologie et a F Einbryogetiic" a 

 work of such excellence and one so well adapted to its purpose 

 (that of instructing the beginner in the preparation of histological 

 material), that the translation is oHered to our readers in the hope 

 that at least some will be thereby induced to study the structure 

 of animal tissues, and that those engaged in teaching the novices 

 in histologv will find its elementarv methods helpful. 'I'he for- 

 muhe and methods given bv M. lioneval arc those which he has 

 proved to be trustworthv, and those which any intelligent begin- 

 ner may prepare and use without further aid. The chemicals 

 needed are usually only such as any good drug-store can supply 

 or any dealer in microscopical materials furnish at small cost. 

 It is assumed that the reader is familiar with the use of the 



