56 THE AMERICAN MONTHLY. llVl.nian, 



hoi. After twentv-foiir hours the tissue is ready tor cleaning, tor 

 whicli herjjamot oil is useil. The object remains in thisfVoin two 

 to twentv-four hours, accordinj; to si/.e, when it is removed to a 

 hath ot' equal parts of solt paratVui and ber^amot oil, kept at the 

 meltinj; point; then to paratlin which melts at 45 C, where it 

 remaitis from one to two hours. The object is now removetl to 

 paratlin which melts at SvS^ C ; after remaininfj from one to two 

 hours it is tinallv imbeilded. The sections are now cut with the 

 microtome, and fixed upon the slide by the modified method 01 

 (iulland. A drop of water is placed on the slide over the surface 

 to be occupied bv the section. The slide is then warmed but 

 kept below the meltinjj point of the paraffin in which the object 

 is imbedded. The sectii)n is placed t)n the drop of water, where 

 it at once flattens perfectly : the excess of water is removed by 

 blottinjj-paper ; the slitle is kept warm until the water has evap- 

 orated. The parartin surroundinLj the section is then cautiously 

 melted. The slide is placeil in xylol or ben/ine to remove paraf- 

 fin, and is then placed in 95 per cent, alcohol, where it remains 

 from an hour and a half to six hours. The sections arc now 

 treated with pin-e tincture of iodine in order to remove the excess 

 of corrosive sublimate ; the tincture is allowed to act for about 

 fifteen minutes; the slide is then placed in 95 ])er cent, alcohol, 

 where it remains until no more iodine dissolvesout of the section, 

 which is then ready for the stain. 



The sublimate fixin*; solution is spoiled by contact with metal- 

 lic substances, and tiss\ie fixed in it becomes brittle if kept \on^ 

 in alcohol. The disadvantaj^es may be overcome by usinj^ ji^lass 

 or wooden rods tor transferring objects to and from the solution, 

 and bv imbedding early. 



Detection of Frozen Meat. — The process adopted by the 

 author tV»r ilistinguisliing l)etween fresh n)eat and that which has 

 been preserved in the frozen state consists in expressing a little 

 blood or meat juice from the sample and examining it under the 

 microscope. The whole operation must be performed quickly, 

 in order to prevent any drying up of the liquid under examina- 

 tion. ■ Wiien the juice of the fresh flesh is thus examined it is 

 seen to contain numerous red corpuscles, which are normal in 

 color, and float in a clear serum. In the case of blood from 

 frozen flesh, the corpuscles have dissolved in the serum under the 

 influence of the low temperature, and not a single normal red 

 corpu.scle can be seen. The hiumoglobin escapes into the serum, 

 and appears as irregular yellow-brown crystals. These may be 

 frequentlv seen bv the naked eye, but, in every case, can be 

 readilv detectetl under the microscope. — Maljcan, in J. Pharm. 

 Chim. 



Cleaning Bottles. — Greasy bottles : Wash with benzine or 

 with a solution of permanganate of potash to wiiicli has been 



