1893.] MICROSCOPICAL JOURNAL. 119 



are destroyed, l)ut this may be avoided by makinf^ sections of 

 sputum which lias been fixed and hardened. For this purpose 

 alcohol, F!emniiM<^'s fluid, chroiiiacetic acid, picric acid, and sat- 

 urated sul>liniate solution arc well suited. Miiller's fluid cannot 

 be used, as it softens and disinte<^rates the masses of expectorant. 



The stainin<^ solutions emplovetl by the author were alum- 

 carmine, satranin, and haniatoxvlin-eosin. By this method, in 

 three cases out of four examined, j^iant cells were demonstrated. 



Tubercle Bacilli Detection. — Dr. SoUes sujj<jests the follow- 

 in<i metlioil tor seaichiiij^ for tubercle bacilli in tissue. Sn)all 

 cubes of the tissue are colored with the two following liquids: 



1. Distilled water, loo parts ; Prussian blue, i part; oxalic acid, 

 2 parts. 



2. Distilled water, loo parts; gelatin, i part. 



The two liquids are mixed and the bits of tissue immersed for 

 a sufficient time. They are then put for 12 hours in absolute 

 alcoiiol. 12 hours in ether, anil the same time in celloidin. By this 

 method the anatomical elements are all colored, while the 

 microbes remain uncolored. lie has studied microbes in cancer 

 bv the same method. 



MT( H()S(()PI(AL NOTES. 



New List of Microscopic Sections of Rock and Minerals. 



— New and interesting specimens from Colorado, Australia, 



New Zealand. Brazil, Hungary, Scotland, Wales, etc., 1-6 each. 



special sets for physiogiaphy. List free. James R. Gregory, 88 



Charlotte vSt., Fizrov Sq.. \V. London. 



Rock Sections for the Microscope. — From 1-6 each : col- 

 lections of minerals for cabinets etc. Thomas D. Russell, 78 

 Newgate St., London. E. C. 



Living Specimens for the Microscope. — Volvox globator, 

 Epistvlis flavicans, .Spongilla fluviatilis ; ova of trout, showing 

 circulation of blood ; Amcrba, Vorticella, Hydra, etc., specimen 

 tube with drawing and description, i s., post free. T. E. Bolton, 

 Farley Road, ^L^lvern Link. 



MICKOISCOPICAL SOCIETIES. 



BlOLOGICAI, AND MICROSCOPICAL SeCTION, PhII.ADKLPHI A 



Academy of Sciences. 

 This section held, during the year 1892, nine meetings, ex- 

 clusive of those in conjunction with the Academy. The member- 

 ship has been increased by one new member, and one name 

 has been added to the list of contributors. Many interesting com- 

 munications have been made, the more important of which are : 

 On Actinomyces bovis^ by Dr. S. G. Dixon ; A Peculiarity in 



