1893.] MICROSCOPICAL JOURNAL. 203 



KUKLRH's NErTItol'HII.K STAIN. 



Distilled WaWr, 100 parts, 



(»ranj;e G, (sjiturated watery solution,) 13."> parts, 



Kubin S, (saturated watery solutiou,) 75 parts, 



Distilled water, 100 parts, 



Absolute Alcohol, 100 parts, 



Methyl (ireen, (sjitunited aqueous solution,) 1'2.') parts, 



.Vcjua distilletl, 100 parts. 



Alcohol, 100 jiarts, 



Glycerine, 7") parts. 



CHENZINSKIE's KOSIX METHYJ.INE HLl'E SOUTIOX, 



Eosin (crystal), 1 per ceut solution in 70 per cent alcohol, 20 parts, 

 Methylene Bine, (saturated watery solution), 40 parts. 



Water di.stilled, 20 parts, 



Glycerine, 20 parts. 



The crystals, used in the preparation of these stains should be 

 strictly of sek'ct(xl quality as it has been found that some of 

 the same kind, but from different manufacturers, produce en- 

 tirely different effects, especially ihe Methylene Blue which is 

 obtainable from the one source only, where Dr. Ehrlicii })ro 

 cures his. 



A brief outline is here given of the manner of using these 

 stains. The steps given are as followed by Dr. Ehrlich. 



Have l)efore you a piece of filter paper on which are i)laced a 

 number of carefully cleaned cover glasses. These must be very 

 thin, "extra No. 1". Wash the cover glasses first in strong 

 suli>huricacid, rinse in water and place them for a few moments 

 in glacial sulphuric acid ; they should then be washed in flow- 

 ing water until all the acid is removed, then transfered to 95 

 per cent alcohol from which they are to be taken and wi])ed. 



Unless the cover glasses are thin and clean no good prei)ara- 

 tioQ can be made. In case the blood is taken from man, the 

 finger is pricked with a steel i)en, one of the prongs of which 

 have been broken off. From the flowing blood a very small 

 drop is caught on a cover glass ne'ar its edge and the glass 

 quickly ])laced, blood side- downward, on another cover glass 

 which should be held in Ehrlich's Blood Cover-Glass Forcep, 

 care being taken to cover the second glass only about one half. 

 The blood will be seen to spread out between the two covers. 

 Quickly draw one cover glass from the other ; a thin layer of 

 blood will in this way be s])read on both slii)S. Ten to twenty 



