252 llii: A.MKKh'AN .M ( >Nri 1 1.^• iScpt., 



!i Jii|K'tt(' tln' s«*|»;irato<l .ind stained (cUs, put a drop (»n the slide, 

 and i»|>|dy the thin (•t)v«'r carefully. 



For (ilanu'iitous tissues, tlie foresroinj; should he modi lied. 

 The hest method is to dissociate l»y line foreeps. A |iieee of 

 tissue iiein;; placed in the dissociating; litpiid, the ends of a iila- 

 mentous fascicle an* sei/.ed hy forceps and slowly torn in two. 

 Tiiis is repeated on the pieces obtained until we have a siumII 

 hundle contuinins; a few lilaments. .Mount on the slide; stain 

 and cover. 



Dissociation by Interstitial Injection. — (Ranvier method). 

 Fill a hyptxlermic syrinjie with the licpiid, either osndc acid (1 

 part to 1U(H)), the i alcohol, or iodised serum. Pierce the organ 

 with tlie canula and force in the injection. If the tissue is com- 

 |»act, separation is finished })y needles or liy forceps, in a small 

 (juaniity of the lluid used ; if the tissue is loose, like subcutane- 

 ous tissue, the injection will form a rounded swelling. With 

 curved scissors cut oil" a hit of the enlargement and add the thin 

 cover. The movements should he rajnd, or the licjuid will How 

 away, the elements become scattered, and the injection will 



have produced no useful (fleet (Ranvier) This is the 



preferable method for studying loose connective tissue. 



1 AkT II. — APPLIED TECHNIQUE. 



Loose Connective Tissue. — The best is the subcuUmeous 

 cellular tissue. Remove a large j)iece of skin, preferably from 

 the fold at the groin where the subcutaneous layer is abundant, 

 and place the subcutiineous layer upward, taking care not to 

 entiingle hairs in it, and with a hypodermic syringe gently in- 

 ject picro-carmine into the tissue. It fornis a rosy swelling 

 . . . of gelatinous consistence. With curved scissors cut off a 

 slice and di.^card it. Cut from the freshly reddened tissue a sec- 

 ond piece as small as possible, rapidly put it on the slide, add a 

 little iticro-carmine, cover and examine. The subcutaneous 

 tissue of tlie dog is well ad:ipfed to this. 



Connective Tissue Bundles. — The foregoing preparation 

 will show these and the elastic fibres. In 2 or 3 hours it will 

 be strongly colored ; . . . then run under the cover a drop of 

 glycerine with 1 per cent of acetic acid. The connective tissue 

 bundles will swell, be decolored and here and there show Heule's 

 sjtiral fibres stained red. 



