1893.] MICROSCOPICAL JOURNAL. 25.3 



Cells. — This luetlntd is insuflicient for the study oltliecon- 

 nective tissue cells. Beautiful preparations of the anastomosiiif^ 

 pr()hnii;ations of the cells may he inadeby injecting, asdescrihed, 

 a mixture of 1 per cent solution of eosin in the h alcohol. Place 

 a small piece on a slide, add a drop of glycerine slightly tinged 

 with eosin, and subject it to gentle i»ressure with a needle. It 

 should be examined with a wide angled ol)jective. 



In the connective tissue which forms the frame-work of or- 

 gans, besides the foregoing flat cells, there are 8i)ecial elemen's 

 called by Erlich granulation cells or plasmatic cells. To ob- 

 serve these proceed thus. The tongue of the frog (where the 

 cells are abundant), the epithelium being scraped off, is put in 

 strong, alcohol for 24 hours, after sj^reading it out on a piece of 

 glass. Then transfer to the following, for 24 hours : Absolute 

 alcohol, 50 c. c; glacial acetic acid, 12 grms., ; distilled water' 

 100 grms.; dahlia, enough almost to saturate the solution. Wash 

 in alcoliol for a few minutes and mount in balsam. All the ele- 

 ments are colorless except Erlich's cells whose protoplasm is 

 stained an intense violet white, the nucleus is not colored. 



Tiie elastic fibres in the connective tissue of organs may be 

 studied in sections of the skin. , . A section, strongly colored 

 on the slide by eosin in alcohol or in water, is treated by a 4(» 

 per cent solution of caustic potash. Cover and examine in the 

 potiish solution. The elastic fibres alone keep tlie rose color. . . 



ADIPOSE TISSUE. 



After an injection into the subcutaneous tissue of nitrate of 

 silver, 1 part to water 10(H) parts, we take shreds of tissue in 

 wliicii to study the constituent elements of the adipose cells. 

 The membrane surrounding the nucleus, the protoplasm and 

 the fat are very a})parent. ... To dissociate the adii>ose tissue, 

 place j)ieces of it to soak in ether. In an hour or two the fat 

 will be dissolved, and the membranes enveloping the vesicles 

 can be seen fallen together and wrinkled so that their i)resence 

 can be readily proved. ... In tliese tissues after death, we find, 

 at the center of each vesicle, not a liquid drop, as in life, but a 

 collection of crystalline needles imperfectly rosette-shaped, and 

 these appear to be crystals of margarine 



Osmic acid and quinoline l»lue should be used as stains. 

 Osmic acid colors fat a deep brown, black if the action is pro- 

 longed. This is characteristii-. Fat cells in the marrow of 



