1893.] MICROSCOPICAL .TOURXAL. 255 



again, and mount in {glycerine. If the exposing to the silver 

 lias been short the endothelium alone is impregnated; if for a 

 longer titne and t<> a stronger solution, the suhi-ndotlu-lial cells 

 will \)v niarki'd out. 



('fo be cunliuucd.) 



Prcparinjf Vejictable Tissue for the Microscope,- Stain in if. 



15V ABRAHAM 1-LATTEK.S 



MANCHESTEU, KNGL.XNI). 



[From Keix)rt of the Manchester Microscopical Society.] 



I.— TO STAIN WITH LOGWOOD. 



After the sections have been bleached and washed : — 1. Place 



them in 70 i>er cent alcohol for 15 minutes. 2. Pour off' the 



alcohol and cover the sections with the stain, and allow them to 



stand from 5 to 30 minutes. 3. Pour off" the stain and rinse the 



sections with 92 per cent alcohol. 4. Pour oil' the alcohol and 



pass the sections through oil of cloves, and mount in balsam 



or dammar. 



II.— TO STAIX WITH PICRO-CAliMINE. 



After the sections have been bleached and washed : — 1. Place 

 them in 92 per cent alcohol for 45 minutes. 2. Pour off' the al- 

 cohol, and cover them with the stain, and allow them to stand 

 from 45 to 60 minutes. 3. Pour off'the stain and rinse the sections 

 with alcohol for two or three minutes. 4. Pour ofi' the alcohol, 

 and cover the sections with an alcoholic solution of })icrate of 

 ammonia, and allow them to stand for one liour. This siiould 

 be renewed and again allowed to stand for 45 minutes. 5. Pour 

 off' the solution and rinse the sections quickh' with 92 per cent 

 alcohol. 6. Pour off' the alcohol, and cover the sections with oil 

 of cloves, and when clear mount in balsam. Rectified spirits and 

 absolute alcohol are sometimes recommended in staining with 

 picro-carmine, but 92 percent methylated alcohol answers very 

 well for this purpose. 



III.— TO STAIN WITH BOKAX-CAKMINE AND lODINK GKEKN. 



After the sections have been bleached and washed : — 1. Place 

 them in 70 per cent alcohol for 15 minutes. 2. I\>uroff"the 

 alcohol, and cover the sections with the stain, and allow them 

 to stand for 45 minutes. 3. Pour off" the stain, and rins < the 

 sections with the acid-alcohol, i.e., one drop of hydrochloric-acid 

 to i oz. alcohol. 4. Pouroll'the aciil-alcohol, and rinse the sec- 



