:V2\ THE AMERICAN MONTHLY [Nov., 



and axis oyliiidors jilso acquiro the stain. Sections nnist l)e 

 very thin. More rectMitly Kuhsoliitzky ha.^ used the following 

 stain : ah-ohol iH> per cent KHt parts, theabove mentioned riihin 

 solution o to oc. cm. With this the st!unin«: ])roce8S is much 

 lenj:ihened (half hour or more), but sections are less apt to break 

 up. 



BACTERIOLOGY. 



New Multiple Staining Fluid.— Dr. P. G. Unna differen- 

 tiates haeilli in tissues by a polyehroniic methylene blue solu- 

 tion, which contains methylene red and violet, in addition to 

 the blue. The sections are transferred from alcohol and allowed 

 to remain in the sUiin for at least ten minutes. They are then 

 passed through water into 33 per cent, tannic acid solution to 

 decolorize, allowed to remain from two to five minutes, then 

 rinsed with water to enable the exact tint to be observed more 

 readily. If satisfactory, after a thorough w-ashing witli water 

 the sections are placed in absolute alcohol, or a solution of gold 

 orange in the same if a yellow counter stain be desired, cleared 

 in oil of bergamot, and mounted in balsam. If the excess of 

 stiiin is not readily removed, a few minutes immersion in 25 per 

 cent nitric acid, followed by dilute spirit, water, and absolute 

 alcohol, respectively, will effect its removal. By adopting this 

 method it is said to be possible to distinguish two kinds of nu- 

 clei (violet and blue), the fibrin, and the protoplasm of the 

 plasma-cells. The bacilli slain red whilst the mucus surround- 

 ing them is blue, and the organisms are said to ai)pear in their 

 natural character " in fish-roe like masses of vegetable mucus." 

 It is claimed that the process is particularly suitable for use in 

 the study of leprosy. It ajipears to depend upon the property, 

 also utilized by Isicolle, by which tannin converts methylene 

 blue into an insoluble form. — Drugghits' Circular and Chemical 

 Gazette. 



MEDICAL MICROSCOPY. 



Trichinoisis in Belgium, — In one town, 13 persons out of 

 39 aflected, recently, died. Lard was found to contain enor- 



