18S9.] MICROSCOPICAL JOURNAL. 31 



Connective tissue stains yellow : epidermis, the rete Alalpighi and 

 the analogous laver in the mucous tissue, muscle, and labial glands 

 •stain light red. the nuclei standing out sharplv. 



Metanil Yellow and Crystal Ponceau [Alphaazonaphthallin- 

 disulfobetanaphthol of soda]. — Preparation: spinal cord of calf, alco- 

 hol hardening. 



For the single as well as the combined stain, twenty-four hours are 

 required. * 



The grav matter stains yellow, the white reddish. Under strong 

 magnification the neurolgia and connective tissue are found to be stained 

 yellow ; the axis cylinders dark bluish red ; the myelin light yellowish 

 red ; one sort of ganglion cells dark purple, another bluish red ; nuclei 

 do not stand out sharp. 



Metanil Yellow and Congo Red [Tetraazodiphenyldinaphthyla- 

 mindisulphonate of soda]. — Preparation: spinal cord of calf, alcohol 

 hardening. 



The sections are stained in a mixture of the staining fluids for S min- 

 utes, or thev are stained for lo minutes in the yellow stain and then tor 

 ^ minutes in the red. 



Ganglion cells [without clear nuclei staining] and axis cylinders stain 

 dark violet-red ; the medullary sheath light citron-yellow ; neurolgia 

 and all connective tissue light violet-red ; epithelium of the central canal 

 brownish-red. 



Carminate of Soda and Metanil Yellow. — The central nervous 

 svstem is hardened in Midler's fluid, then stained in toto with the car- 

 mine fluid. Sections are then stained for ten minutes in the yellow 

 stain. 



All nervous elements are stained red. all connective tissue elements 

 xellow. 



Crystal Ponceau and Crystal Violet. — Preparation : transverse 

 section of the carotid of the calt\ alcohol hardening. 



The sections are stained for 5 minutes in the red ponceau fluid and 

 then for i minute in the violet. 



Nuclei of the endothelium and smooth muscle stain violet: all the 

 other tissues red. 



Congo Red and Anisol Red [Bisulfoxylnatronbetaoxynapthalina- 

 zoorthometoxvlbenzol]. — Preparation: spinal cord of the calf, alcohol 

 hardening. 



The sections are stained for 5 minutes in the combined stains, or for 

 :; minutes in the Congo red solution and then for 5 minutes in the anisol 

 red . 



Axis cvlinders and cell bodies stain purple ; all other tissues stain 

 light red. Nuclei do not stain. 



Metanil Yellow and Athylin Blue. — Preparation: ala nasi of a 

 child, alcohol hardening. 



When the two staining solutions are combined a black precipitate is 

 formed, which re-dissolves in an excess of the metanil vellow solution. 

 This solution stains yellowish-green, the cartilage only being stained 

 blue If the sections are first stained for 5 minutes in a mixture of 5 

 c.c. of the vellow and 4 c.c. of the blue stains, or if the sections are 

 stained for ten minutes in the yellow and then for 2 minutes in the blue, 

 the pictures will be sharp. 



