8 THE AMERICAN MONTHLY [Jan., 



newly sterilized utensils are put into the box and ar- 

 ranged in a manner which experience will indicate. Then 

 the box is again closed. The hands of the worker are 

 carefully washed, and the slide-door is raised so that it 

 allows the hands and a part of the arms to conveniently 

 work inside. A small acohol-lamp may be kei)t in the 

 box for the final disinfection of needles, covers, slides, 

 etc. But everything should be carefully sterilized before 

 being put in. 



This box is very convenient for a great deal of myco- 

 logical work. It was first used by .the famous physio- 

 logist at the Carlsberg Laboratory, Dr. Em. Clir. Hansen. 



It should be added that a small cross-bar (a, a) prevents 

 dust from falling into the box at the top of the sliding- 

 door. 



FURTHER STUDIES. 



Pursuing the case before us we find that both of the 

 two forms of cells which suggested the identification of 

 cultivated yeast and one of the wild forms have formed 

 spores after a time of 40 hours by 25.° The spore-cul- 

 tures are taken out of the thermostat, and the cover 

 lifted while a sample of the yeast layer at the top of the 

 gypsum bloc is scraped off" with a needle. This sample 

 is then microscopically examined. In this case a diam. 

 magn. of about 8-900 should be used. Seibert's oc. 3, 

 obj. 3 always gives splendid pictures. Referring to Fig. 

 2, we find some cells with partition-walls and spores, in- 

 dicating the presence of S. ceremsioi I. Other cells of 

 this yeast have no such walls (a) ; all these spores look, 

 however, pale o^* " emptied." The other spore-bearing 

 cells bear spores of a more refractive appearance, smaller 

 than those mentioned above ; these (b) evidently belong 

 to one of the wild forms ; the number of spores as well 

 as the form of their mother-cell points towards one of 

 the /S. pastorianitfi. At c we find a spore-bearing cell of 



