1894.] MICROSCOPICAL JOURNAL. 9 



/S*. eereciske I with spores just being formed, and at d, 

 we perceive the same feature of the spore-foruiation of 

 the other form. 



At present it is still an open question to which species 

 the wild form belongs; this must be decided by a, pure 

 culture. The carrying out of a pure culture will be 

 described in the second part of this paper. 



We add two more examples of yeast-analysis before 

 turning towards other questions. 



Fig. 4 shows the microscopical picture of another sam- 

 ple. This time the mixture of different organisms is 

 artificial. The analysis was given to the writer in Jor- 

 gensen's laboratory in Copenhagen. The figure was 

 drawn from Seibert oc. 2, obj. 3, and shows two distinct 

 types of yeast fungi cells, some round or oval, and some 

 of a pastorian form. The clear, homogeneous protoplasm 

 of the former indicates a culture yeast. At b we have a 

 septated mycelium and cells with large oil drops ; these 

 might belong to a Monilia or Chalara, or similiar forms. 



After 40 hours, some of the yeast cells had formed 

 spores, the appearance of which (see above) show thatAS*. 

 cerevisiae is present. The cells that contained no spores 

 had aggregated protoplasm. This shows that all of the 

 yeast present is belonging to the above named species. 



Much septated mycelium was found on the spore cul- 

 ture. A moist chamber culture from this, in a hanging 

 drop of beer-wort, showed a typical Monilia Candida. 



Finally, we have another sample, the microscopic pic- 

 ture of which is demonstrated in Fig. 5. We find here 

 (a) round or oval cells with homogenous protoplasm, like 

 in a cultui'e yeast"^^, (b) lemon shaped cell^, some of whicli 

 have a round bud at its end. This is undoubtedly Reess" 

 Saccharoniyces apiculatusf whose cells can always be 



*Here, we are unable to say whether it is a variety of S. cerev. I, or not. 

 The qnestiou of variation in this species is far-reaching, and has not yet been 

 fuUv debated. 



+This is one of Keess' .species from 1870. Spores were never found in the 



