1896.] MICROSCOPICAL JOURNAL. 135 



Collagen, orcein red ; muscle, protoplasm, bluish ; 

 keratin, nuclei, plasma cell, blue ; prickle cells, methy- 

 lene red. 



(b) Acid FiLchsin- Picric Method. 



1. 2 per cent acid fuchsin solution, 5 minutes. 



2. Water. 



3. Concentrated watery solution of picric acid, 1 min- 

 ute. 



4. Concentrated spiritous solution of picric acid, 1 

 minute. 



5. Absolute alcohol, oil, balsam. 



Collagen, keratin, nuclei, red; muscle, protoplasm yel- 

 low. 



(c) Methylene- Blue-Permanganate of Potassium, Mur- 

 iatic Acid- Alcohol Method. 



1. Polychrome methylene- blue, 10 minutes. 



2. Water. 



3. 1 per cent solution of permanganate, 10 minutes. 



4. Water. 



5. Acid alcohol (1 per cent HCl), 10 minutes. 



6. Absolute alcoliol, oil, balsam. 



Collagen, decolorized; muscle, protoplasm, nuclei, vio- 

 let. 



II. Comparison of Smooth Muscle with Collagen and 



Elastin. 



(d) A cid Orcein-Hemati7i-Acid Fiichsin -Picric Method. 



1. Acid orcein solution, 10 minutes while being heated. 



2. Wash in diute spirits. 



3. Strong hematin solution, 10 minutes. 



4. Decolorization of the collagen in acid alcohol a few 

 seconds. 



5. Water. 



6. 2 per cent acid fuchsin solution, 5 minutes. 



7. Concentrated watery solution of picric acid, 2 min- 

 utes. 



