Vlll 



TIME SCHEDULE OF LESSONS 



SUBJECT OF LESSON 



VIII. BACTERICIDAL TESTS IN 

 VITRO. 



IX. AGGLUTININS. 



X. AGGLUTINATION MICRO- 

 SCOPIC METHOD. EF- 

 FECT OF SALT. 



XI. ABSORPTION OF AGGLUTI- 

 NINS. 



XII. IN Vivo EFFECT 6r H^EM- 

 AGGLUTINATIVE AND 

 H^MOLYTIC SERA. 



XIII. ISOH^MOLYSIS AND IsO- 



AGGLUTINATION. 



XIV. PRECIPITINS. 



XV. FORENSIC 

 TEST. 



PRECIPITIN 



XVI. BORDET-GENGOU PHENOM- 

 ENON. 



XVII. ALEXIN FIXATION BY SPE- 

 CIFIC PRECIPITATES. 



XVIII. FORENSIC COMPLEMENT 

 FIXATION. 



XIX. WASSERMANN ANTIGEN : 

 PREPARATION AND Ti- 



TRATION OF ANTIGEN. 



XX. WASSERMANN REACTION : 

 TECHNIQUE OF TESTS. 



XXI. COMPLEMENT FIXATION 

 WITH BACTERIAL EX- 

 TRACTS. 



WORK PRELIMINARY TO SUBSE- 

 QUENT LESSONS 



Titrate hemolytic sera made by 

 class. 



Inoculate flasks with virulent diph- 

 theria strain for production of 

 diphtheria toxin (Lesson XXII). 



Filter diphtheria toxin (Lesson 

 XXII). 



Begin preparation of Wassermann 

 antigens for Lesson XIX. 



Inject guinea pigs with diphtheria 

 toxin for determination of mini- 

 mal lethal dose (Lesson XXII). 



Finish preparation of simple alco- 

 holic Wassermann antigen and 

 Noguchi antigen. 



Inject guinea pigs with diphtheria 

 toxin-antitoxin mixtures for de- 

 termination of L dose (Lesson 

 XXII). 



