10 LABORATORY COURSE IN SERUM STUDY 



For injection a syringe is used which will withstand boiling. 

 For general work all glass syringes of the Luer type are the most 

 satisfactory. A tightly fitting needle of gauge 20 to 22 should be 

 used for "Inrra venous injections; for intraperitoneal injections 

 larger needles may be used if desired. If glass syringes are used 

 the needle may be attached to the barrel of the syringe, the 

 plunger withdrawn, and the two parts boiled separately for 5 

 minutes in water containing about 1 per cent of sodium carbonate. 

 A pair of forceps should be placed in the sterilizer with the points 

 under the water. The barrel is first removed with the forceps, 

 the plunger then removed and inserted. The fluid to be injected 

 is drawn into the syringe by tilting the test tube or container 

 and placing the beveled tip of the needle closely against the 

 lower side of the wall. If air is drawn into the syringe at the 

 same time, it should be expelled by holding the syringe vertically, 

 needle up, covering the tip of the needle with a small piece of 

 cotton wet with alcohol or some other disinfectant and expelling 

 the air. After use the syringe should be resterilized if any 

 infected material has been used, washed with water, and the 

 needle washed with water, then with alcohol and then with ether 

 to dry it thoroughly. If care is not taken to clean the needles 

 thoroughly after use, they are certain to become clogged. If blood 

 or serum have been injected, the needle and syringe must be 

 cleaned with cold water at once. Otherwise if alcohol or boiling 

 water is used, the protein will be coagulated and the needle clogged. 

 Animals should be labeled by attaching a number tag by means 

 of a metal staple to the ear, should be placed in a cage with a tag 

 recording the number of the animal, the name of the student in 

 charge and the nature of the material injected, and the date of 

 each injection. 



SCHEME FOR INJECTIONS (APPROXIMATE DOSES AND INTERVALS) 

 1. Rabbit immunized to B. typhosus by intravenous injection. 

 1st day 1st dose 0.05 agar slant 

 6th day 2d dose 0.1 agar slant heated as 



llth day 3d dose 0.2 agar slant indicated 



16th day 4th dose 0.2 agar slant below 



24th day 5th dose 0.2 agar slant 



