14 LABORATORY COURSE IN SERUM STUDY 



6. Rabbit immunized to sheep serum by intravenous injection. 

 Five-day intervals. 



1st dose 2.0 c.c. 

 2d dose 2.0 c.c. 

 3d dose 2.0 c.c. 



7. Rabbit immunized to human serum (ascitic fluid) by intravenous 

 injection. Five-day intervals. 



1st dose 4.0 c.c. 

 2d dose 4.0 c.c. 

 3d dose 4.0 c.c. 



8. Rabbit immunized to horse serum. (Immunize noteless than 

 three in order to obtain sufficient serum for later exercises.) 



1st dose 2.0 c.c. 

 2d dose 2.0 c.c. 

 3d dose 2.0 c.c. 



9. Rabbit immunized to sheep erythrocytes by intravenous injec- 

 tion. Four or five day intervals. 



1st dose 4.0 c.c. of 50 % suspension 

 2d dose 4.0 c.c. of 50 % suspension 

 3d dose 4.0 c.c. of 50 % suspension 



A fourth injection may be given in some cases, but is not often 

 necessary. 



10. Rabbit immunized to sheep erythrocytes by intraperitoneal 

 injections. Intervals as above. 



1st dose 4.0 c.c. of 50 % suspension 

 2d dose 8.0 c.c. of 50 % suspension 

 3d dose 8.0 c.c. of 50 % suspension 

 4th dose 8.0 c.c. of 50 % suspension 



11. Rabbit immunized to cat erythrocytes by intravenous injec- 

 tions of 2 c.c. at 4-day intervals. See Lesson XII. 



A preliminary titration of the serum done with a specimen 

 obtained from an ear vein should be done about the eighth or 

 ninth day after the last injection. If this is satisfactory, the 

 bleeding of the animal is usually done the ninth or tenth day. No 

 absolute rule can be established as to exact period at which the 

 serum has the highest antibody concentration, this depending to 



