C6 



LABORATORY COURSE IN SERUM STUDY 



Add saline 

 to 2 c.c. 

 and then 

 0.5 c.c. of 

 the red cell 

 emulsion. 1 



Set up the following tests : 



Series 1 



Immune Serum (Sensitizer) Complement 



Tube 1. (diluted 1-100) 0.5 c.c. 0.05 c.c. 



2. (diluted 1-100) 0.2 c.c. 0.05 c.c. 



3. (diluted 1-1000) 1.0 c.c. 0.05 c.c. 



4. (diluted 1-1000) 0.5 c.c. 0.05 c.c. 



5. (diluted 1-1000) 0.4 c.c. 0.05 c.c. 



6. (diluted 1-1000) 0.3 c.c. 0.05 c.c. 



7. (diluted 1-1000) 0.2 c.c. 0.05 c.c. 



8. (diluted 1-1000) 0.1 c.c. 0.05 c.c. 



9. (diluted 1-1000) 0.0 c.c. 0.05 c.c. 



(Complement Control) 



Series 2 



Immune Serum (Sensitizer) Complement 



Tube 1. (diluted 1-100) 0.5 c.c. 0.02 c.c. 



2. (diluted 1-100) 0.2 c.c. 0.02 c.c. 



3. (diluted 1-1000) 1.0 c.c. 0.02 c.c. Add saline 



4. (diluted 1-1000) 0.5 c.c. 0.02 c.c. to 2 c.c. 



5. (diluted 1-1000) 0.4 c.c. 0.02 c.c. and then 



6. (diluted 1-1000) 0.3 c.c. 0.02 c.c. 0.5 c.c. of 



7. (diluted 1-1000) 0.2 c.c. 0.02 c.c. the red cell 



8. (diluted 1-1000) 0.1 c.c. 0.02 c.c. emulsion. 



9. (diluted 1-100) 0.5 c.c. 0.00 c.c. ( 



(Sensitizer Control) 



Series 3 



Immune Serum (Sensitizer) Complement 



Tubel. (diluted -100) 0.5 c.c. 0.01 c.c. 



2. (diluted -100) 0.2 c.c. 0.01 c.c. 



3. (diluted -1000) 1.0 c.c. 0.01 c.c. Add saline 



4. (diluted -1000) 0.5 c.c. 0.01 c.c. to 2 c.c. 



5. (diluted -1000) 0.4 c.c. 0.01 c.c. and then 



6. (diluted -1000) 0.3 c.c. 0.01 c.c. 0.5 c.c. of 



7. (diluted 1-1000) 0.2 c.c. 0.01 c.c. the red cell 



8. (diluted 1-1000) 0.1 c.c. 0.01 c.c. emulsion. 



9. (diluted 1-1000) 0.0 c.c. 0.00 c.c. J 



(Salt Solution Control) 



1 A half c.c. instead of one c.c. is used in order to save materials. The amounts 

 of complement and amboceptor needed are of course half those that would be needed 

 for 1 c.c. of cells. 



