84 LABORATORY COURSE IN SERUM STUDY 



1. Serum (1-10) 0.5 c.c. + Typhoid suspension 0.5 c.c. 



2. Serum (1-10) 0.5 c.c. + Colon suspension 0.5 c.c. 



3. Serum (1-50) 0.5 c.c. + Typhoid suspension 0.5 c.c. 



4. Serum (1-50) 0.5 c.c. -f Colon suspension 0.5 c.c. 



5. Serum (1-200) 0.5 c.c. + Typhoid suspension 0.5 c.c. 



6. Serum (1-200) 0.5 c.c. + Colon suspension 0.5 c.c. 



7. Serum (1-1000) 0.5 c.c. + Typhoid suspension 0.5 c.c. 



8. Serum (1-4000) 0.5 c.c. + Typhoid suspension 0.5 c.c. 



9. Salt solution 0.5 c.c. + Typhoid suspension 0.5 c.c. 

 10. Salt solution 0.5 c.c. + Colon suspension 0.5 c.c. 



Incubate one hour at 37 C. and observe. 



(2) Set up the following tests with the same serum, from which 

 in this case, however, the agglutinins have been removed by ab- 

 sorption : 



1. Serum absorbed with typhoid 0.5 c.c. + Typhoid suspension 0.5 c.c. 



2. Serum absorbed with typhoid 0.5 c.c. + Colon suspension 0.5 c.c. 



3. Serum absorbed with colon 0.5 c.c. + Typhoid suspension 0.5 c.c. 



4. Serum absorbed with colon 0.5 c.c. + Colon suspension 0.5 c.c. 



5. Serum absorbed with colon 



in the highest dilution 

 which agglutinated 

 typhoid bacilli before 

 absorption 0.5 c.c. + Typhoid suspension 0.5 c.c. 



Incubate for one hour and observe. 

 Each student needs: 



25 J-inch test tubes, 8 1-c.c. pipettes, test tube rack, 35 -inch 

 test tubes, 4 centrifuge tubes, test tube racks. 



LESSON XII 



EFFECT OF HJEMOLYTIC AND H^EM AGGLUTINATIVE 



SERA IN VIVO 



1. PRELIMINARY TESTS 



A RABBIT receives at four-day intervals four intravenous injections 

 of 2 c.c. of washed, cat's red blood cells. For obtaining the cells a cat 

 under ether is bled from the carotid or jugular. The defibrinated cat's 

 blood if sterile can be kept four days and thus used for two injections. 

 Seven to ten days after the last injection the rabbit is bird and the 

 hsemolytic and agglutinative power of the inactivated immune serum for 

 cat's cells is tested. 



