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LABORATORY COURSE IN SERUM STUDY 



Each student tests one specimen of serum against 10 different indi- 

 viduals' red cells. Make a control of each serum with its own red cells. 

 Lay all the pipettes on their sides on a tray and incubate at 37.5 C. 

 for one hour. Then make observations for haemolysis or agglutination 

 and make the results of all the tests into a table to illustrate Land- 

 steiner's law of the groupings of human isoagglutination. 



By the original classification of blood groups the groupings may be 

 arranged as follows on the assumption that there are two distinct ag- 

 glutinins and corresponding agglutinogens: 



From this table the student can determine the grouping of his tests. 

 It will be noted that any individual can be grouped by testing his cells 

 against sera of groups II and III. Study this out on the table above. 

 Every serologist should know his own group. 



The students should carry out a few simplified tests by mixing a 

 drop of blood suspension and a drop of serum on a slide. The slide 

 method is sufficient for all practical purposes. 



The class needs: 



Heavy glass tubing 7-8 mm. diameter. 

 Heavy glass tubing 4-5 mm. diameter. 



Litre 1 per cent sodium citrate in 0.6 per cent sodium chloride. 

 Litre 0.9 per cent sodium chloride solution. 

 Triangular files. 



Rubber nipples to fit 5 mm. tubing (one nipple for each student). 

 Hard paraffine in casserole. 



