110 



LABORATORY COURSE IN SERUM STUDY 



meat with 5 c.c. of salt solution and filtering. The clear solution 

 represents approximately a 1-100 dilution of the protein. From 

 it a 1-1000 dilution should be prepared. 



The following experiment should then be set up : 



All tubes are to be brought up to a volume of 1.5 c.c. by the addition 

 of salt solution. 



Incubate for one hour at 37.5 C. 



At the end of this time 0.5 c.c. red cells, sensitized with two units 

 of amboceptor, are added to each tube and the tubes are again incubated 

 until haemolysis is complete in controls "5," "6," "7" and "8." 



The purpose of the control tubes "5" and "6" is, of course, to prove 

 that the antigen alone without the presence of the antiserum does 

 not interfere with the complement action of the guinea pig serum, while 

 the purpose of the control number "7" is to make certain that the 

 antiserum alone does not interfere with the action of the complement. 

 Tube "8" is put in for a similar purpose so that a slight inhibitory 

 action (anticomplementary effect) on the part of either antigen or anti- 

 serum can be recognized by contrast with the complete laking which 

 ensues from the unhampered effect from the guinea pig serum on the 

 sensitized red cells. 



MATERIALS 



Each student requires : 

 5 grams of each of 2 types of 

 chopped meat (horse and any 

 other variety). 



2 hard filter papers and funnels. 

 1 c.c. antihorse serum 

 5 c.c. guinea pig serum (1-10). 

 20 units antisheep-cell amboceptor. 



8 one-c.c. pipettes. 



8 half-inch test tubes. 



2 large test tubes or wide mouth 



bottles. 



1 test tube rack 

 5 c.c. sheep cells. 

 Salt solution. 



