172 LABORATORY COURSE IN SERUM STUDY 



Experiment 1 

 HEAT SUSCEPTIBILITY OF NORMAL OPSONIN 



Divide the normal serum obtained from own finger into two parts. 

 Heat one of these in a water bath at 56 C. for 30 minutes. 



Now carry out opsonin tests in parallel (1) with unheated serum, 

 (2) with the heated serum using same bacterial emulsion and leuco- 

 cytes in both cases. 



Compare results by counting slides. 



Experiment 2 

 HEAT SUSCEPTIBILITY OF IMMUNE OPSONIN 



Staphylococcus immune rabbit serum will be given out. 

 Divide into two parts. Heat one part to 56 C. for 20 minutes in 

 a water bath. 



Carry out parallel tests as above and compare the results. 



Materials : 



Staphylococcus emulsion. 



Glass tubing for Wright capsules and pipettes. 



Staphylococcus immune serum. 



LESSON XXIX 

 TITRATION OF NORMAL OPSONIN BY DILUTION METHOD 



TITRATE opsonic power of normal guinea pig serum for Staphylo- 

 coccus aureus by dilution method as follows : 



1. Make dilutions of serum 1-5, 1-10, 1-20, 1-100. 



2. Set up series of opsonic tests in Wright capillary pipette, using 

 Staphylococcus suspension, suspension of guinea pig leucocytes and 

 diluted serum equal parts. Make additional test with undiluted serum, 

 and also control with leucocytes, bacteria and salt solution. 



3. Incubate 30 minutes, make smears and stain with Jenner's stain. 

 Determine by counts in which dilution there is a definite increase of 

 phagocytosis as compared with the salt solution control. 



Materials : same as for two preceding lessons. 



