184 LABORATORY COURSE IN SERUM STUDY 



Materials needed for each group of students : 



4 guinea pigs. 



1 c.c. horse serum. 



1 c.c. sheep serum. 



Syringe. 



Scalpel, forceps, scissors, thread, for isolation and ligation of jugu- 

 lar vein. 



Ether. 



LESSON XXXIII 

 ANAPHYLAXIS (Continued) 



II. PASSIVE SENSITIZATION 



OBTAIN serum of rabbit which has been highly immunized to horse 

 serum. 



Make preliminary titration of precipitin of this serum for horse 

 serum. 



Inject 1.0 c.c. of this serum intravenously into each of three guinea 

 pigs. After 24 hours inject into the opposite jugular vein as follows : 



Guinea pig (a) 0.1 c.c. horse serum 

 Guinea pig (6) 0.3 c.c. horse serum 

 Guinea pig (c) 1.0 c.c. horse serum 



Observe symptoms. 



In case of anaphylactic death, immediately open chest and observe 

 condition of lungs and also whether heart is still beating. While such 

 an animal is dying it is important to watch carefully the gradual altera- 

 1 tion of respirations, the great irregularity and slowing of breathing and 

 the intense inspiratory efforts just before death. 



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