TECHNIQUE OF INOCULATION. 9 



For this purpose, lysol, lysoform and cresol solutions are highly recommend- 

 able. Sublimate is less efficient because it coagulates albumins and thus 

 may lead to plugging of pipettes which may have contained blood rests. If 

 highly infectious material has been examined, it is best to place the entire 

 disinfectant solution containing the used glassware into the autoclave, 

 sterilize it there, then wash the supply thoroughly with soap, dry and resteril- 

 ize it by dry heat for i to 2 hours at 120 C. 



The Technique of Inoculation. 



Both for serum diagnosis and serum therapy, the serum is required from 

 animals which have been artificially immunized against the bacteria or 

 their products of secretion. Almost without exception, this immunization 

 is produced by injecting the animal with the infectious virus. The method 

 of inoculation is either intravenous, w/raperitoneal, or subcutaneous. 



The technique of intravenous injection varies somewhat with 

 Intravenous different animals. In rabbits, the veins running along the 

 Injection. outer margins of the ears are most suitable. The assistant 

 sits upon a chair, holds the hind legs and body of the rabbit 

 tightly fixed between his knees and thus has his hands free to steady the 

 rabbit's ears. Another method consists in placing the rabbit upon the table 



FIG. 3. Intravenous inoculation. (After Uhlenhuth.} 



and firmly holding him there while the injection is made (Fig. 3). The ear 

 is first struck gently with the fingers and washed with alcohol and xylol. 

 If the hair is very long, it should be clipped. If the vein running along the 

 outer margin of the ear is exceptionally small, it can be made more promi- 

 nent by compressing it between the thumb and index finger at the root of the 



