12 



LABORATORY EQUIPMENT. 



SUBCUTANEOUS inoculation is the simplest of all methods. 



Subcutaneous A fold of skin is elevated between the thumb and index finger 



Injection, of the left hand and the needle plunged into the subcutaneous 



tissue. In rabbits and guinea-pigs the skin of the back or 



abdomen is chosen, as the subcutaneous tissue here is not tense. In goats, 



sheep, and horses the skin of the neck and shoulder region is preferred. 



I $i>- 



FIG. 5. Removal of peritoneal exudate. Guinea-pig held in Friedberger's position. (Origi 



The skin of the back and abdomen is to be avoided because following the 

 injection edema frequently arises, which may extend to the lower extremities 

 and thus interfere with locomotion. 



If abscesses arise after subcutaneous injection, they should be opened, 

 washed out with lysol solution and dressed with iodoform. 



The Methods of Obtaining and Preserving Serum. 



Venesection or venous puncture is the method best adapted for obtaining 

 blood from animals. The veins employed for that purpose are those which 



