TECHNIQUE OF ANTIRABIC VACCINATION. 



subcutaneously some fresher spinal cord, i.e. (dried for a lesser period of time), until 

 finally he used virus dried only for one day. The animals thus treated were immune 

 against the bites of rabid dogs as well as subdural, subcutaneous, and intravenous 

 infection with "virus fixe" and street virus. This procedure was strongly recommended 

 by Pasteur, who brilliantly contributed the observation, that if an animal was infected 

 but did not as yet show symptoms, these could be prevented by a similar modus 

 operandi, as above mentioned. 



In man, the inoculation is carried out on the same principle. The 

 fact that the incubation period of hydrophobia is very long, makes the pro- 

 phylactic inoculations of greater service. Only rarely is this period less 

 than six weeks, usually considerably longer up to 584 days, entirely depend- 

 ent upon the virulence of the virus and the point of infection. 



Technique of Antirabic Vaccination in Man. 



The actual vaccine consists of i c.c. (2-3 mm. length) of the substance of 

 the spinal cord of a rabbit which has been killed by inoculation with the 

 fixed virus, rubbed up into a fine emulsion with 5 c.c. of sterile 0.85 NaCl 

 solution. About i to 3 c.c. of the resulting fluid are injected subcutaneously 

 into the skin of the abdomen. A cord dried for fourteen days is used for 

 the first injection, emulsions of less attenuated virus are used on succeeding 

 occasions until finally a portion of a spinal cord dried for only three or four 

 days is employed. Pasteur's schemes of the actual doses can thus be drawn 

 up. 



a. For infections at points distant from the central nervous system (mild infections}. 



b. For head wounds (severer infections}. 



