FIRST FUNDAMENTAL AGGRESSIN TEST. 



35 



A small incision is made in one of the intercostal spaces on the side of 

 the chest, and through this wound a long canula is introduced into the 

 pleural cavity. Following the injection, the animal as a rule, rapidly 

 succumbs to the infective organism. On autopsy, the pleural cavity is 

 found to contain an exudate of a reddish-brown color (hemorrhagic) on 

 the side where the inoculation was given, and of yellow serous on the other 

 side. This bloody exudate measuring about 15 c.c. is removed^ with a 

 sterile pipette, placed into a sterile centrifuge tube to which is added 1.5 

 c.c. of 5 per cent, carbolic acid drop by drop (making the entire solution a 

 1-2 per cent, carbolic acid dilution) , agitated continually in order to prevent 

 precipitation, and followed by centrifugalization at a very high speed for 

 many hours until it becomes very clear. The upper clear part which is 

 now free of bacteria, or very nearly so, is drawn off by a pipette and heated 

 for three hours at 44 C. Its sterility is then tested and if no growth 

 appears after forty-eight hours, it is considered sterile. 



First Fundamental Aggressin Test. 



(Its power of increasing severity of infections.} 



