12 LABORATORY COURSE IN SERUM STUDY 



2. Rabbit immunized to B. typhosus by subcutaneous injections. 

 Injections at five or six day intervals. 



1st dose 0.1 agar slant 

 2d dose 0.2 agar slant 

 3d dose 0.3 agar slant 

 4th dose 0.5 agar slant 

 5th dose 0.5 agar slant 



3. Rabbit immunized to B. typhosus and to B. coli communior by 

 intravenous injection. Intervals as above. 



1st dose 0.05 agar slant of B. typhosus 

 2d dose 0.1 agar slant of B. typhosus 

 3d dose 0.2 agar slant of B. typhosus 

 4th dose 0.2 agar slant of B. typhosus, plus 1 



0.05 agar slant of B. coli communior J 

 5th dose 0.2 agar slant of B. typhosus, plus 1 



0.05 agar slant of B. coli communior J 

 6th dose 0.2 agar slant of B. typhosus, plus 1 



0.1 agar slant of B. coli communior J 

 7th dose 0.2 agar slant of B. typhosus, plus 1 



0.2 agar slant of B. coli communior J 



4. Guinea pig immunized to Sp. cholerae by intraperitoneal injec- 

 tion. An old laboratory strain of the cholera vibrio should be used 

 for immunization. Intervals as above. 



1st dose 0.05 agar slant 

 2d dose 0.1 agar slant 

 3d dose 0.2 agar slant 

 4th dose 0.2 agar slant 

 5th dose 0.2 agar slant 



5. Rabbit immunized to micrococcus aureus by intravenous injec- 

 tion. Intervals as above. In the case of this microorganism the 

 intervals should be lengthened if there is any indication of illness. 

 Great care is necessary in avoiding overdosage. 



1st dose 0.05 agar slant 

 2d dose 0.1 agar slant 

 3d dose 0.2 agar slant 

 4th dose 0.2 agar slant 

 5th dose 0.2 agar slant 



