30 LABORATORY COURSE IN SERUM STUDY 



Tube 1 



Supernatant fluid 1.5 c.c. 



Fresh guinea pig serum 1-10 (complement) 0.5 c.c. 

 Washed sheep cells 5 % .0.5 c.c. 



Tube 2 



Supernatant fluid 1.5 c.c. 



Immune rabbit serum 0.5 c.c. 



Washed sheep cells 5 % 0.5 c.c. 



TubeS 



Sediment 2.0 c.c. 



Guinea pig serum 1-10 0.5 c.c. 



Tube 4 



Sediment 2.0 c.c. 



Immune rabbit serum ........ 0.5 c.c. 



Incubate one hour at 37 C., observing at frequent intervals. 



The results will show that the haemolytic amboceptor or sensitizer 

 present in the original heated immune serum was bound by the cells at 

 C. but at this low temperature the complement or alexin remained 

 not only inactive but unbound and free. 



APPARATUS 

 Each student will require : 



3 graduated centrifuge tubes 

 16 half-inch test tubes 



9 one c.c. pipettes 



1 test tube rack 



1 bottle of salt solution 



1 jar of cracked ice (pint measure) 



1 rubber nipple and capillary pipette 



The class will require : 

 1 outfit for bleeding sheep : 



needle, 



tourniquet, 



razor and scissors, 



bottle of tincture of iodine, 



sterile flask containing beads. 



