36 



LABORATORY COURSE IN SERUM STUDY 



Set up the following tests : 



Immune 



Tube 1. (diluted 



2. (diluted 



3. (diluted 



4. (diluted 



5. (diluted 



6. (diluted 



7. (diluted 



8. (diluted 



9. (diluted 



Series 1 

 Serum (Sensitizer) 



1-100) 0.5 c.c. 

 1-100) 0.2 c.c. 

 1-1000) 1.0 c.c. 

 1-1000) 0.5 c.c. 

 1-1000) 0.4 c.c. 

 1-1000) 0.3 c.c. 

 1-1000) 0.2 c.c. 

 1-1000) 0.1 c.c. 

 1-1000) 0.0 c.c. 



Series 2 



Immune Serum (Sensitizer) 



Tube 1. (diluted 1-100) 0.5 c.c. 



2. (diluted 1-100) 0.2 c.c. 



3. (diluted 1-1000) 1.0 c.c. 



4. (diluted 1-1000) 0.5 c.c. 



5. (diluted 1-1000) 0.4 c.c. 



6. (diluted 1-1000) 0.3 c.c. 



7. (diluted 1-1000) 0.2 c.c. 



8. (diluted 1-1000) 0.1 c.c. 



9. (diluted 1-1000) 0.0 c.c. 



Series 8 

 Immune Serum (Sensitizer) 



Tube 1. (diluted 1-100) 0.5 c.c. 



2. (diluted 1-100) 0.2 c.c. 



3. (diluted 1-1000) 1.0 c.c. 



4. (diluted 1-1000) 0.5 c.c. 



5. (diluted 1-1000) 0.4 c.c. 



6. (diluted 1-1000) 0.3 c.c. 



7. (diluted 1-1000) 0.2 c.c. 



8. (diluted 1-1000) 0.1 c.c. 



9. (diluted 1-1000) 0.0 c.c. 



Complement 



0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 

 0.05 c.c. 



Add saline 

 to 2 c.c. 

 and then 

 0.5 c.c. of 

 the red cell 

 emulsion. l 



Complement 



0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 

 0.1 c.c. 



Add saline 

 to 2 c.c. 

 and then 

 0.5 c.c. of 

 the red cell 

 emulsion. 



Complement 



0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 

 0.2 c.c. 



Add saline 

 to 2 c.c. 

 and then 

 0.5 c.c. of 

 the red cell 

 emulsion. 



1 A half c.c. instead of one c.c. is used in order to save materials. The amounts 

 of complement and amboceptor needed are of course half those that would be 

 needed for 1 c.c. of cells. 



