66 



Examine a dried specimen of blood for this reaction. 

 Add a drop of bouillon to the drop of dried blood on a slide 

 and after it has become well mixed add a loopful of it to a 

 similar quantity of a fresh bouillon culture and examine it im- 

 mediately in a hanging-drop. 



108. Securing blood for the Widal test, (i) Dried 

 preparations. From a prick in the finger or lobe of the ear (if 

 a lower animal the shaved ear is a good place) sufficiently deep 

 to procure a drop of blood which is placed on a slide by means 

 of a platinum loop, and allow it to dry. (2) Serum. From a 

 similar but deeper prick or by drawing a few drops of blood 

 from a vein with a hypodermic syringe secure a few drops of 

 blood, place them in the bottom of a small, short sterile tube 

 and allow the serum to ooze out. This can often be helped by 

 separating the blood from the tube by means of a sterile wire. 

 If retained for any length of time before making the test 

 the serum must be kept in a cool place. 



EXERCISE XXXV. 



BACTERIUM (BACILLUS) SEPTICAEMIAS HEMORRHAGICAE 

 AND MICROCOCCUS LANCEOLATUS. 



109. Explanatory note. These organisms are the 

 causes of swine plague or infectious pneumonia in swine and 

 of croupous or lobar pneumonia in man. 



The name Bacillus septicaemiae hemorrhagicae was given 

 by Hiippe to the bacillus of swine plague (Smith). This 

 bacterium is morphologically and in its cultural characters 

 not distinguishable from the bacillus of rabbit septicaemia, 

 (Koch), bacillus of fowl cholera, (Pasteur), and of Schweine- 

 seuche, (Schutz). It is similar to a species of pathogenic 

 bacteria found more or less frequently in the upper air pass- 

 ages of nearly all of the domesticated animals. It is very 



