68 



stained with alkaline methylene-blue, carbol fuchsin and an 

 aqueous solution of methyl-violet. 



Describe the appearance of the bacteria and make a draw- 

 ing of a few of them from one preparation. 



Preserve a preparation of each species to accompany 

 notes. 



If there is any growth in the gelatin tube make a series of 

 3 gelatin plates from the bouillon culture. 



EXERCISE XXXVII. 



BACTERIUM (BACILLUS) SEPTICAEMIAS HEMORRHAGICAE 

 AND MICROCOCCUS LANCEOLATUS (continued). 



112. General directions. Re-examine all the cultures 

 of these bacteria paying special attention to the reactions of 

 the liquid cultures. 



Make the indol test with the cultures in Dunham's solu- 

 tion. 



Make, stain, and examine a cover- glass preparation from 

 an organ or the blood of a rabbit which has died from the 

 inoculation with swine plague bacteria, and also a prepara- 

 tion made from a rabbit which has died from the inoculation 

 with Micrococcus lanceolatus . Stain the preparations with an 

 aqueous solution of fuchsin. Study the bacteria in these 

 preparations and carefully compare the two. Indicate in 

 the notes the differences, if any are found. 



Keep the cultures until the next exercise and compare 

 them again, after which they may be rejected. 



