EXERCISE XXXIX. 



BACTERIUM (BACILLUS) MALLEI. 



117. Explanatory note. This organism grows most 

 characteristically on potato, and somewhat feebly in the 

 other media heretofore used. It develops readily on acid 

 agar, and in acid glycerine agar and acid glycerine bouillon. 

 For this reason it is not inoculated into the full set of media. 

 In diagnosing glanders, it is customary to inoculate guinea- 

 pigs with the suspected material. From the lesions in these 

 animals, if the disease develops, pure cultures can usually be 

 .obtained. It can be identified from its morphologic and 

 cultural characters. 



118. General directions. Inoculate a tube of potato, 

 one of agar, one of acid agar, one of acid glycerine agar, one 

 of glucose agar and one of bouillon from a culture furnished. 

 (The special media here introduced will be furnished by the 

 instructor). 



119. The preparation of acid agar. This is prepared 

 the same as ordinary agar ( 16) with the omission of the 

 sodium hydrate in the bouillon from which it is made. 



1 20. The preparation of acid glycerine agar. Add 

 5% glycerine to acid agar before sterilizing it. 



EXERCISE XL. 



BACTERIUM (BACILLUS) MALLEI, (continued). 



121. General directions. Examine and carefully de- 

 scribe all the cultures of Bacterium mallei. 



Make three cover-glass preparations from the acid agar 

 and from the bouillon cultures, and stain one of each with 

 alkaline methylene-blue, one with carbol fuchsin and one 



