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EXERCISE 



DETERMINE THE SPECIES OF BACTERIA. 



132. Explanatory note. The two cultures of bacteria 

 assigned belong to species already studied. The student 

 should identify the species of the bacteria in the cultures. 

 To do this such media should be inoculated and such micro- 

 scopic examinations made as he thinks necessary. The 

 notes should contain a complete record of the work and the 

 reasons for the identifications made. 



133. General directions. Identify the bacteria in the 

 cultures assigned at the last exercise. 



EXERCISE XLVII. 



ISOLATING AND IDENTIFYING BACTERIA FROM ANIMAL 



TISSUES. 



134. Explanatory note. In making a bacteriological 

 investigation into the cause of death in an animal or man it 

 is necessary to make cultures from the various organs and 

 the blood to find whether or not there are any pathogenic or 

 other bacteria present. This necessitates a knowledge of 

 making cultures from animal tissues. In this exercise an 

 experimental animal (rabbit or guinea-pig) will be provided 

 which has died from some bacterial disease. The purpose 

 of this examination is to find out what that disease is. To 

 save animals each student will make cultures from but one 

 organ. Opportunity will be afforded from time to time 

 during the course for making cultures from various animal 

 tissues. 



135. General directions. The experimental animal 

 will be furnished tied out on a post-mortem tray and the 



