79 



EXERCISE XLVIII. 



ISOLATING AND IDENTIFYING BACTERIA FROM ANIMAL 

 TISSUES (Continued). 



137. General Directions. Examine and describe all of 

 the cultures made from the animal tissues. 



Examine the bouillon and agar cultures microscopically in 

 the fresh condition and in stained cover-glass preparations. 



If the species can not be determined from these cultures 

 and examinations, make such others from the agar culture as 

 may be necessary to do so. Examine these at the next ex- 

 ercise when the notes can be completed. 



State in the notes the facts upon which the identification 

 is based. 



EXERCISE XLIX. 



THE EXAMINATION OF SECTIONS OF TISSUE CONTAINING 

 BACTERIA. 



138. Explanatory note. The preparation of tissues for 

 sectioning and the study of the tissue changes more properly 

 belong in the course in pathology. It is important, however, 

 that one is able to distinguish bacteria in the lesions which 

 they produce. For this reason an exercise is devoted to the 

 study of bacteria in sections of tissues already stained and 

 mounted. These include the various pneumonias, tubercu- 

 losis, anthrax, hog cholera, typhoid, septicaemia, etc. 



139. General directions. Examine the sections fur- 

 nished for the bacteria and note especially their distribution 

 in the tissues. Make drawings of a few of the bacteria from 

 each preparation. 



