SECTION i 

 LABORATORY RULES 



1. Familiarize yourself with the laboratory rules. 

 Upon their careful observance depend good work and 

 your own safety. 



2. Food must not be eaten in the laboratory; pencils, 

 labels, or fingers must not be moistened with the 

 tongue. 



3. If any portion of a culture is spilt by accident 

 upon the desk or floor, it should be covered immediately 

 with a germicide (HgCl 2 1:1000, or carbolic acid in 

 5 per cent solution). After this germicide has acted 

 for 10 or 15 minutes, wipe it up and throw the cloth or 

 paper into a waste jar. 



4. In case the hands should come in contact with 

 infectious material, they should be washed with one 

 of the above mentioned germicides, and then scrubbed 

 with soap and water. 



5. The platinum needles used in making cultures 

 should be sterilized in a flame before and after use, and 

 before they are laid down. When the needles are 

 covered with viscous material, as milk, for instance, 

 they should be held at the side of the flame until dry 

 before being sterilized. This will avoid the danger of 

 scattering infectious material about the desk. 



6. All possible care should be observed in handling 

 apparatus, etc. Solid material should not be put into 

 sinks. Burned matches, paper, cotton, broken glass, 



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