BACTERIOLOGICAL TECHNIC 17 



for one hour. This process has to be repeated for five 

 or six successive days, before sterilization is complete. 

 If the autoclave is to be used, the tubes are placed 

 in the autoclave in an inclined position. 

 Good results are obtained by this method: 



FIG. n 

 Koch Inspissator 



1. Close the lid and the steam outlet. 



2. Admit steam. After reaching 3 pounds pressure, 

 keep this pressure for 5 minutes. 



3. Increase the pressure slowly to 5 pounds and 

 keep there for 5 minutes. 



